FIG. 5.

Antimycobacterial activity of representative clones against infected macrophages and the effects of specific inhibitors. Bone marrow-derived macrophages were infected with M. tuberculosis H37Rv (about four bacteria/macrophage) and then incubated with representative clones at an E/T ratio of 50:1. MAbs against Fas or FasL were added, or the clones were degranulated with Sr²⁺ (23) before the effectors and targets were mixed. After 24 h at 37°C, the cells were fully lysed by adding saponin, and the CFU were counted. The bars show means ± SD (n = 3) for representative clones and a nonspecific CD4⁺ CD8⁻ control. Clone 4.4 represents the most common phenotype among CD4⁺ CD8⁻ clones, and clone 8.6 represents the most common phenotype among CD4⁻ CD8⁺ clones (Fig. 1).