Skip to main content
. 2022 Dec 16;8(50):eabq6720. doi: 10.1126/sciadv.abq6720

Fig. 2. Creation and validation of the hiPSC line with R531W mutation in KCNH2.

Fig. 2.

(A) Trace files of the target site in wild-type (WT) (left) and edited (right) hiPSCs, showing a heterozygous edit in c.C1591T, resulting in R531W. (B) Immunostaining of pluripotency markers (where SOX2 is in red and NANOG is in green), (C) karyotype analysis, (D) potential off-target loci sequencing, and (E) trilineage potential of the hiPSC with R531W colony, revealing maintenance of hiPSC characteristics after gene editing. Scale bars, 50 μm (B) and 100 μm (E).