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. 2022 Dec 16;8(50):eabq6720. doi: 10.1126/sciadv.abq6720

Fig. 5. Defective trafficking of hERG in CMs differentiated from hiPSCs with R531W mutation.

Fig. 5.

(A) Western blot for hERG1a and (B) densitometry analysis of 135 to 155 kDa bands of WT and edited hiPSC-CMs. (C) Densitometry analysis of 135 to 155 kDa bands of WT and edited hiPSC-CMs after 24 hours of exposure to E-4031, and (D) the change in ratio of 135 to 155 kDa hERG after exposure to E-4031. (E) Immunostaining of TNNT2 (green), hERG (red), and 4′,6-diamidino-2-phenylindole (DAPI) (blue), and (F) just hERG (red) and DAPI (blue) in WT and edited hiPSC-CMs. Scale bars, 50 μm. *P < 0.05.