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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: Adv Nanobiomed Res. 2022 Sep 14;2(10):2200052. doi: 10.1002/anbr.202200052

Figure 5.

Figure 5.

Schematic of the decellularization approach. Cells (iPS rejuvenated iDFU) are seeded for 3 weeks (with 100μg/mL ascorbic acid (AA)) allowing ECM to be deposited directly in place prior to decellularization. This should leave an ECM enriched scaffold with unaltered coated rejuvenated elements to be tested for DFU seeding. Decellularization takes place in 2 subsequent steps: Freeze thaw in ddH2O followed by lyophilization (1st step) and rehydration in ddH2O and gDNAse (2nd step) washings. These scaffolds are considered decellularized (D-iDFU or D-DFU). The resulting scaffolds are then crosslinked a second time and sterilized for analysis and seeding.