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. 2022 Dec 6;11:e80859. doi: 10.7554/eLife.80859

Figure 1. A cohort-based experimental design.

A cohort-based experimental design can facilitate determining the correlation between desiccation resistance, cuticular hydrocarbons (CHCs), and body weight. Five to six cohorts of each species were established.

Figure 1.

Figure 1—figure supplement 1. The phylogeny of 50 Drosophila and related species used in this study.

Figure 1—figure supplement 1.

Fifty species representing 46 Drosophila species (18 from Drosophila subgenus, 28 from Sophophora subgenus) and 4 species (3 Scaptodrosophila species and 1 Chymomyza species) as outgroup were used in our study. The 46 Drosophila species represent some of the main groups in the Drosophila genus: (1) melanogaster group, (2) obscura group, (3) willistoni group, (4) nasuta group, (5) virilis group, and (6) repleta group.
Figure 1—figure supplement 2. Chromatogram of the authentic standard n-alkane mixture which contains 34 n-alkanes from C7 to C40 at the same concentration.

Figure 1—figure supplement 2.

The authentic standard n-alkane mixture (C7–C40; Supelco 49,452U) was obtained from Sigma-Aldrich (St. Louis, MO). C7–C14 were excluded in the chromatogram due to the solvent delay in the gas chromatography–mass spectrometry (GC–MS) program. The integration of peak areas on longer cuticular hydrocarbons (CHCs) is biased to have lower values. To address this issue, we corrected the peak areas of CHCs based on the carbon-chain lengths using the integration from this standard n-alkane mixture.