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. 2022 Dec 5;29(12):1217–1227. doi: 10.1038/s41594-022-00864-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 4 — a, Flowchart of the transcription read-through assay. b, Representative traces of RNAP encountering a bound dCas9 from the PAM proximal side. An example control trace is shown with RNAP and bound dCas9 detected at their expected locations. After NTP addition, shown are example traces of RNAP prior to encountering the dCas9 (top) and RNAP colliding with dCas9 (bottom). c, Representative traces of transcription assays with dCas12a in the PAM-Distal orientation. An example control trace is shown with RNAP and bound dCas12a detected at their expected locations. After NTP addition, shown are example traces of RNAP prior to encountering the dCas12a (top), RNAP colliding with dCas12a (middle), and RNAP reading through dCas12a (bottom). d, Representative traces of RNAP encountering a bound dCas12a from the PAM proximal side. An example control trace is shown with RNAP and bound dCas12a detected at their expected locations. After NTP addition, shown are example traces of RNAP prior to encountering the dCas12a (top) and RNAP colliding with dCas12a (bottom). Source Data File for plots in b-d is provided.

Source data