Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 5;29(12):1217–1227. doi: 10.1038/s41594-022-00864-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, RNAP collision with dCas9 from the PAM-proximal side without dCas9 removal. Top, an example unzipping trace. Bottom, force peak locations of RNAP and dCas9. The dashed lines indicate the expected RNAP force peak position when RNAP contacts dCas9 (Extended Data Fig. 1a). Each box plot represents the 25th–75th percentiles of force peak positions of n biologically independent traces with error bars indicating s.d.: n = 88 (3-nt mismatch), n = 114 (unmodified), n = 103 (5-nt inverted repeat (IR)), n = 108 (6-nt IR) and n = 103 (7-nt IR). b, RNAP collision with dCas9 from the PAM-distal side without dCas9 removal. Top, an example unzipping trace showing the stalled RNAP and dCas9 force peaks. Bottom, force peak locations of RNAP after collision with dCas9. Each box shows the 25th–75th percentiles of force peak position distribution with error bars indicating s.d.: n = 14 (3-nt mismatch), n = 77 (unmodified), n = 83 (5-nt IR), n = 157 (6-nt IR) and n = 163 (7-nt IR). c, RNAP collision with dCas9 from the PAM-distal side with dCas9 being removed. Top, an example unzipping trace showing the stalled RNAP force peak with the dCas9 force peak being absent. Bottom, force peak locations of RNAP and dCas9. Each box shows the 25th–75th percentiles of force peak position distribution with error bars indicating s.d.: n = 23 (3-nt mismatch), n = 22 (unmodified), n = 12 (5-nt IR), n = 15 (6-nt IR) and n = 12 (7-nt IR). d, Efficiency of dCas9 removal with different gRNA modifications. The percentage with removal due to transcription read-through (gray, from Fig. 4c) and the percentage with removal but without read-through (red) are stacked. The percentage dCas removal values were calculated for each sample chamber (black dots), and the mean value and s.e.m. of these repeats are also shown. The same data as in Fig. 4c were used for this analysis, so the sample statistics are identical to those for Fig. 4c. Source data for a–d are provided.

Source data