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. 2022 Nov 24;29(12):1159–1169. doi: 10.1038/s41594-022-00857-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 3 — a-e) On the left - FSC plot showing the overall resolution of the reconstructions determined by the gold standard FSC cut-off 0.143, indicated in the graph. In the middle – angular distribution plot of the respective reconstruction showing assignment of particles with respect to various angles. Colour bar indicates number of samples per angular bin (white areas indicate unpopulated angles). On the right - Model-to-map FSCs, showing the fit of modelled structures to their corresponding maps.