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. 2022 Nov 24;29(12):1159–1169. doi: 10.1038/s41594-022-00857-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — Extended Data Fig. 8a) Birds-eye view of the SNAPc interaction with the GTFs’ and the PSE motif on U5 snRNA promoter. The dashed boxes indicate the observed interaction surfaces within the complex (1–4). b) Structural super-position of ideal B-DNA of U5 promoter to the SNAPc bound experimental DNA structure. Major and minor grooves of U5 promoter bound by SNAPC3 and SNAPC4are labelled and highlighted with lines. Dashed box indicates the PSE region. c) Close up view of SNAPC3 helix α8 binding to major groove of U5 promoter. The observed steric clash of K194 with B-DNA highlights the distortion upon SNAPc binding. d) Close up view of SNAPC3 helices α4, α5 region binding to minor groove of U5 promoter. The views in panels c and d correspond to Fig. 4b, c. e) Sequence logos of DNA sequence surrounding TSS peaks in expressed constitutive first/single exons for all snRNA genes (n = 18) and protein coding genes (n = 4721), sorted by TSS precision scores. The boxes indicate the IMR region (−8 to +2) of promoter flanking the TSS ( + 1). While the protein coding genes do not show any enrichment of specific nucleotides, snRNA genes present a AT-rich profile in the IMR region, indicating its tendency for spontaneous promoter opening.