Extended Data Fig. 1. Cryo-EM structure determination of microtubule-associated filaments of LRRK2^RCKW[I2020T] in the presence of MLi-2.

a, Optimization of in vitro reconstituted microtubule-associated LRRK2^RCKW filaments. Top row, cryo-EM images of an individual microtubule (left) or individual microtubule-associated LRRK2^RCKW filaments. Middle, Diffraction patterns calculated from the images above. Arrowheads point to layer lines arising from the microtubule (white) or from the LRRK2^RCKW filaments (open), and their frequencies are indicated below the images. Bottom, 2D class averages from multiple images equivalent to those shown at the top. The type of LRRK2^RCKW (WT, G2019S, or I2020T) and the presence or absence of MLi-2 during filament reconstitution are indicated at the top. b, Schematic of data processing pipeline used to obtain the different reconstructions of the microtubule-associated LRRK2^RCKW[I2020T] filaments in the presence of MLi-2 (see Methods for details). Local resolution maps, Fourier Shell Correlation, directional FSC plots, and the distribution of voxel resolutions are shown for all reconstructions discussed in the text.