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. 2022 Oct 12;60(1):84–97. doi: 10.1007/s12035-022-03057-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Characterization of Ren-derived astrocytes after digoxin exposure. A Diagram of the culture and treatment procedure. B Resazurin viability assay for astrocytes exposed to different concentrations of digoxin for 24 h. Results are displayed as violin with data points, for each group n = 7 samples obtained in 2 independent experiments. Statistical analysis for the effects of digoxin was performed using the non-parametric Kruskal–Wallis test followed by Dunn’s multiple comparison test. C Confocal images of immunostaining for GFAP, vimentin, and S100B a nuclei are stained with Hoechst (blue)