Lectin-based assay identifies GLUT1 as plasma membrane L-fucose transporter.A, schematic showing both de novo and salvage fucosylation pathways. B, schematics of siRNA lectin assay workflow. C, saturation of AAL staining in increasing concentrations of exogenous L-fucose. Each point represents mean from three independent measurements ± SD. D, top hits identified during the siRNA assay exhibiting decreased AAL binding. TT-Score (Two-Target) is the ratio between lectin fluorescence signal and nuclei staining. FCSK, SLC35C1, and FPGT were used as controls. AAL, Aleuria aurantia lectin.