Figure 4.

³H-L-fucose and¹⁴C-2-deoxy-D-glucose uptake in DLD-1 WT and GLUT1 KO cells.A, Western Blot comparison of GLUT1 expression in DLD-1 WT and GLUT1 KO cells. B, comparison of three μM³H-L-fucose and ¹⁴C-2-deoxy-D-glucose uptake in DLD-1 WT and GLUT1 KO cells in a presence and absence of GLUT1 (100 μM BAY-876) and macropinocytosis inhibitors (500 μM Amiloride). C, amount of ³H-L-fucose and ¹⁴C-2-deoxy-D-glucose taken up by various cell lines. D, amount of ¹⁴C-2-deoxy-D-glucose taken up by various cell lines in a presence and absence of 50 μM fucose. Two-tailed, unpaired t test was used to assess statistical significance (ns > 0.05; ∗ ≤ 0.05; ∗∗ ≤ 0.01; ∗∗∗ ≤ 0.001; ∗∗∗∗ ≤ 0.0001). Each bar represents mean from three independent measurements ± SD.