Figure 2.

Tissue distribution and mRNA expression profiles of GLUT1 and GLUT2 in oriental river prawns.A and B, the tissue distribution of GLUT1 and GLUT2 mRNA. RNA samples were extracted from healthy prawns, and GLUT1 and GLUT2 expression was studied using qRT–PCR (with β-actin as the internal reference gene). Shown are the means ± standard error (SE; n = 6). Different lowercase letters indicate the significance (determined using one-way ANOVA). C and D, qRT–PCR analysis of the mRNA expression profiles of GLUT1 and GLUT2 in hemocytes under hypoxia (1% O₂), using β-actin as the reference gene. Shown are the means ± SE (n = 6). ∗∗p < 0.01, ∗p < 0.05 (Student’s t test). GLUT, glucose transporter; qRT–PCR, quantitative RT–PCR.