Fig. 4.

Endothelial cell activation focusing on membrane expression. EA.hy926 cells were stimulated with FFP (n = 65) considered as the gold standard versus the CCP without (n = 65) or with ARs (n = 48). EA.hy926 cells were stimulated with negative and positive controls. TNF-α stimulation was considered the positive control (n = 18) and unstimulated EA.hy926 cells were viewed as the negative control (n = 19). Bioactivity in EA.hy926 endothelial cells was measured with FCM focusing on membrane expression of CD54 (A) and CD31 (B) with or without stimulation. Scatter dot plots represent the data and the red line denotes the median of raw data. Statistical analyses were performed using one-way Anova with multiple comparisons.