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. 2022 Dec 5;18:100512. doi: 10.1016/j.mtbio.2022.100512

Fig. 11.

Fig. 11

Effect of rotenone on PEVs treated pathological NP cells. (A) Effects of the inhibitor rotenone on the fluorescence expression of mitochondrial functional protein PGC1α in each group. Scale bar ​= ​50 ​μm. (B) Quantification of specific immunofluorescence intensities according to (A). (C) Western blot analysis showing the effects of rotenone on SIRT1, PGC1α, and TFAM protein levels in pathological NP cells. βactin was used as loading control. Uncropped gel is in SI Fig. 4F. (D) Quantitative analysis of mitochondrial functional protein levels in each group. (E) Detection of lactic acid concentration in supernatant of pathological NP cells culture medium. (F) ATP levels of pathological NP cells was detected. Significance between four groups was calculated using one-way ANOVA with Tukey's post-hoc test. Data are presented as mean ​± ​SD (n ​= ​3). (Scale bars are listed above; ∗: P ​< ​0.05, ∗∗: P ​< ​0.01, ∗∗∗: P ​< ​0.005, and ∗∗∗∗: P ​< ​0.001).