Table 1.
Gene Name | Used for | Primer sequence | Product length | Amplicon Size |
Col2α | mRNA quantification | F: CAGGATGCCCGAAAATTAGGG | 21 | 132 |
R: ACCACGATCACCTCTGGGT | 19 | |||
Sox9 | mRNA quantification | F: GAGCCGGATCTGAAGAGGGA | 20 | 151 |
R: GCTTGACGTGTGGCTTGTTC | 20 | |||
Acan | mRNA quantification | F: CCTGCTACTTCATCGACCCC | 20 | 150 |
R: AGATGCTGTTGACTCGAACCT | 21 | |||
Mmp13 | mRNA quantification | F: CTTCTTCTTGTTGAGCTGGACTC | 23 | 173 |
R: CTGTGGAGGTCACTGTAGACT | 21 | |||
Mmp3 | mRNA quantification | F: ACATGGAGACTTTGTCCCTTTTG | 23 | 192 |
R: TTGGCTGAGTGGTAGAGTCCC | 21 | |||
Adamts5 | mRNA quantification | F: GGAGCGAGGCCATTTACAAC | 20 | 110 |
R: CGTAGACAAGGTAGCCCACTTT | 22 | |||
βactin | mRNA quantification | F: CCTCTATGCCAACACAGT | 18 | 230 |
R: AGCCACCAATCCACACAG | 19 | |||
P16(Cdkn2a) | mRNA quantification | F: GTGTGCATGACGTGCGGG | 18 | 127 |
R: GCAGTTCGAATCTGCACCGTAG | 22 | |||
P21(Cdkn1a) | mRNA quantification | F: CCAGGCCAAGATGGTGTCTT | 20 | 103 |
R: TGAGAAAGGATCAGCCATTGC | 21 |