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. 2022 Dec 5;18:100512. doi: 10.1016/j.mtbio.2022.100512

Table 1.

Summary of PCR primers used.

Gene Name Used for Primer sequence Product length Amplicon Size

Col2α mRNA quantification F: CAGGATGCCCGAAAATTAGGG 21 132
R: ACCACGATCACCTCTGGGT 19
Sox9 mRNA quantification F: GAGCCGGATCTGAAGAGGGA 20 151
R: GCTTGACGTGTGGCTTGTTC 20
Acan mRNA quantification F: CCTGCTACTTCATCGACCCC 20 150
R: AGATGCTGTTGACTCGAACCT 21
Mmp13 mRNA quantification F: CTTCTTCTTGTTGAGCTGGACTC 23 173
R: CTGTGGAGGTCACTGTAGACT 21
Mmp3 mRNA quantification F: ACATGGAGACTTTGTCCCTTTTG 23 192
R: TTGGCTGAGTGGTAGAGTCCC 21
Adamts5 mRNA quantification F: GGAGCGAGGCCATTTACAAC 20 110
R: CGTAGACAAGGTAGCCCACTTT 22
βactin mRNA quantification F: CCTCTATGCCAACACAGT 18 230
R: AGCCACCAATCCACACAG 19
P16(Cdkn2a) mRNA quantification F: GTGTGCATGACGTGCGGG 18 127
R: GCAGTTCGAATCTGCACCGTAG 22
P21(Cdkn1a) mRNA quantification F: CCAGGCCAAGATGGTGTCTT 20 103
R: TGAGAAAGGATCAGCCATTGC 21