Table 1.
Forward (‐F) and Reverse (‐R) Primer Sequences Used for RT‐qPCR Amplification of Human Genes
| Human gene | Sequence (5’→3’) | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|
| hIL1β‐F | AATCTCCGACCACCACTACAG | 174 | 62 |
| hIL1β‐R | TCCCATGTGTCGAAGAAGATAG | ||
| hIL6‐F | AATGAGGAGACTTGCCTGGTG | 143 | 61 |
| hIL6‐R | AGGAACTGGATCAGGACTTTTG | ||
| hIL8‐F | GATTTCTGCAGCTCTGTGTG | 154 | 61 |
| hIL8‐R | TCTGTGTTGGCGCAGTGTGG | ||
| hTNFα‐F | AGGGACCTCTCTCTAATCAGC | 168 | 61 |
| hTNFα‐R | TCTCAGCTCCACGCCATTGG | ||
| hRPLP0‐F | AGGCTTTAGGTATCACCACT | 219 | 61 |
| hRPLP0‐R | GCAGAGTTTCCTCTGTGATA | ||
| hRPS17‐F | TGCGAGGAGATCGCCATTATC | 170 | 61 |
| hRPS17‐R | AAGGCTGAGACCTCAGGAAC |