TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant features | Reference or source |
|---|---|---|
| Serovar Typhimurium | ||
| LB5010 | Wild type; fimbriate with complete fim gene cluster | 8 |
| LBY100 | LB5010 fimY::kan Kanr | This study |
| ISF145 | LB5010 λfimA lacZ lysogen | 47 |
| ISF145Y | LBY100 λfimA lacZ lysogen | This study |
| SL1344 | Wild type; fimbriate with complete fim gene cluster | 28 |
| SL1344JTY | SL1344 fimY::kan Kanr | This study |
| SL1344JTZ | SL1344 fimZ::kan Kanr | This study |
| E. coli | ||
| SY327 | Host for suicide vector pGP704 | 39 |
| JM109 | Host for fimY lacZ reporter plasmids and FimY expression plasmid; Δ(lacZ) | 53 |
| Plasmids | ||
| pISF101 | fimAICDHFZYWU | 49 |
| pISF145 | fimA-lacZ reporter fusion | 47 |
| pISF182 | fimZ and fimY cloned into pACYC184; FimZ+ FimY+ | 54 |
| pISF187 | pISF182 with translation terminator inserted into fimY; FimZ+ FimY− | 54 |
| pISF189 | pISF182 with translation terminator inserted into fimZ; FimZ− FimY+ | 54 |
| pISF215 | fimY cloned into pACYC184; FimY+ | This study |
| pISF217 | pISF215 with translation terminator inserted into fimY; FimY− | This study |
| pISF234 | fimY-lacZ reporter fusion | This study |
| pISF237 | Single-copy fimY-lacZ reporter fusion | This study |
| pISF241 | fimY-malE fusion for FimY purification | This study |