TABLE 4.
Expression of β-galactosidase by fimY-lacZ reporter constructs in E. coli and serovar Typhimurium
| Strain | Plasmid (relevant genotype) | β-Galactosidase expressiona |
|---|---|---|
| E. coli JM109 | pISF237 (fimY lacZ) | 0 |
| + pISF187 (fimZ+ fimY)b | 0 | |
| +pISF189 (fimZ fimY+) | 0 | |
| + pISF182 (fimZ+ fimY) | 69.4 ± 1.1 | |
| Serovar Typhimurium | ||
| SL1344 | pISF237 | 52.1 ± 2.3 |
| SL1344JTY | pISF237 | 0 |
| SL1344JTZ | pISF237 | 0 |
a
β-Galactosidase activity is reported in Miller (38) units, and the data represent the mean ± standard deviation for one culture assayed in triplicate. All experiments were performed independently at least three times with ≤20% variation.
b
pISF237 plus pISF187.