Fig. 2. Time and concentration courses of UII treatment on TNF-α-R mRNA expression from tunica adventitia of vessels and AFs. After rat tunica adventitia of vessels and AFs were treated with UII (1×10 - 10 to 1×10 - 7 mol/L) for the indicated time, tissues and AFs were collected. Total RNA was extracted and reverse transcription into cDNA for RT-PCR and qPCR was carried out. The data are presented as mean±standard error of the mean (SEM), n=3. * P<0.05, ** P<0.01 vs. 2 h at the same concentration. UII: urotensin II; TNF-α-R: tumor necrosis factor-α (TNF-α) receptor; mRNA: messenger RNA; AFs: adventitial fibroblasts; cDNA: complementary DNA; RT-PCR: reverse transcription-polymerase chain reaction (PCR); qPCR: quantitative real-time PCR.
