Fig. 3. Effects of different inhibitors on UII-induced TNF-α-R mRNA and TNF-α expression in tunica adventitia of vessels and AFs. After preincubation with PD98059 (1×10 - 5 mol/L), Y-27632 (1×10 - 5 mol/L), H-7 (1×10 - 5 mol/L), CSA (1×10 - 5 mol/L), or nicardipine (1×10 - 5 mol/L) for 30 min, the rat tunica adventitias of vessels and AFs were incubated with UII (1×10 - 8 mol/L) and the inhibitors for 24 h. Tissues and AFs were collected, total RNA was extracted, and reverse transcription into cDNA for RT-PCR and qPCR were carried out. Medium was collected for ELISA. The data are presented as mean±standard error of the mean (SEM), n=3. ** P<0.01 vs. control; # P<0.05, ## P<0.01 vs. UII group. UII: urotensin II; TNF-α-R: tumor necrosis factor-α (TNF-α) receptor; mRNA: messenger RNA; AFs: adventitial fibroblasts; CSA: cyclosporin A; cDNA: complementary DNA; RT-PCR: reverse transcription-polymerase chain reaction (PCR); qPCR: quantitative real-time PCR; ELISA: enzyme-linked immunosorbent assay.
