Fig. 6. Effects of APN on UII-induced phosphorylation of AMPK in AFs. AFs were stimulated with APN, UII, compound C (COM), and adipoR RNAi fragments for 24 h. The AFs were analyzed for phosphorylation of AMPK by western blotting. Data are expressed as mean±standard error (SE), n=3. ^** P<0.01 vs. control; ^## P<0.01 vs. UII+gAd. APN: adiponectin; UII: urotensin II; AF: adventitial fibroblast; AMPK: adenosine 5'-monophosphate (AMP)‍-activated protein kinase; p-AMPK: phospho-AMPK; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; gAd: globular adiponectin; AdipoR: adiponectin receptor; RNAi: RNA interference; siRNA: small interference RNA.