Fig. 2.

Decomposition of the T cell compartment in children and adults with COVID-19.

(A) UMAP showing 171,393 CD4⁺ T cells and 127,069 CD8⁺ T cells from children, non-ICU, and ICU adults during Acute and Convalescent phases.

(B) Expression of genes associated with the naïve, interferon response (IFN), T central memory (TCM), T cell cytotoxicity, regulatory T cell (Treg) and activation states by different subclusters of CD4⁺ and CD8⁺ T cells shown in the UMAP.

(C) Detection of interferon-induced MX-1 protein in subpopulations of CD4⁺ T cells during Acute and Convalescence. Gating are as follows for Naïve T cells = CD45RA⁺CD45RO⁻; TCM = CD45RA⁻CD45RO⁺CCR7⁺CD62L⁺; TEM = CD45RA⁻CD45RO⁺CCR7⁻CD62L⁻; TEMRA = CD45RA⁺CD45RO⁻CCR7⁻CD62L⁻.

(D) Detection of interferon-induced MX-1 protein in subpopulations of CD8⁺ T cells during Acute and Convalescence. T cell markers are as in (C).

(E) Detection of interferon-activated naïve CD4⁺ T cells (top) and CD8⁺ T cells (bottom) in children, non-ICU, and ICU adults during Acute and Convalescent phases (left) and in healthy age and sex-matched donors in the OneK1K cohort (right).