Supplementary Fig. S2.

Flow cytometric analysis of PBMCs in children and adults with COVID-19. Gates for each population are as follows:

(A) NK cells. CD3-CD56+

(B) NK bright cells. CD3-CD56bright NK

(C) NK dim cells. CD3-CD56dim NK

(D) Naïve CD4+ T cells. abTCR+gdTCR-CD4+ CD45RA + CCR7+

(E) Memory CD4+ T cells. abTCR+gdTCR-CD4+ CXCR5-CD45RA-

(F) Naïve CD8+ T cells. – abTCR+gdTCR-CD8+ CD45RA + CCRA7+

(G) CD8+ central memory T cells (TCM). abTCR+gdTCR-CD8 + CD45RA- CCR7+

(H) CD8+ effector memory T cells (TEM). abTCR+gdTCR-CD8 + CD45RA-CCR7-

(I) CD8+ terminally differentiated effector memory T cells (TEMRA). abTCR+gdTCR-CD8 + CD45RA + CCR7-

(J) Naïve B cells. CD3-CD20 + CD21 + CD27-CD10-

(K) Memory B cells. CD3-CD20 + CD21 + CD27+

(L) Plasmablasts. CD56-CD3-CD4-CD8-CD19 + CD38hiCD27+.