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FACS gating strategy for OX40 assay and T cell proliferation assay. (A) Representative FACS plot showing gating strategy for detection of CD25+CD134+CD4+ T cells upon stimulation with nil (top panels) and Spike trimer (bottom panels).(B) Representative FACS plot showing gating strategy for proliferation of CD4+ T cells upon stimulation with nil (top panels), and Spike trimer (bottom panels).
