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. 2022 Dec 17;23:356. doi: 10.1186/s12931-022-02234-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — HOTAIR facilitates HPVEC apoptosis via DNMT1 mediated hypermethylation of Bcl-2 promoter in the mouse model. a HE staining of lungs from the control, CSE, CSE + Si-HOTAIR, CSE + Vector and CSE + Si-HOTAIR + OE-DNMT1 groups. Scale bar = 100 μm. b Morphometric measurements of MLI (μm) and DI (%) were performed in each group. Results are expressed as mean ± SD. *P < 0.05 compared with the control group. c Immunoblotting was conducted in lungs from the control, CSE, CSE + Si-HOTAIR, CSE + Vector and CSE + Si-HOTAIR + OE-DNMT1 groups. d–g The relative expressions of DNMT1, Bcl-2, Bax, and Cleaved-caspase 3 in lungs. Results are expressed as mean ± SD. *P < 0.05 compared with the control group. h–k Expression of HOTAIR, DNMT1, Bcl-2 and Bax were measured by qRT-PCR from the control, CSE, CSE + Si-HOTAIR, CSE + Vector and CSE + Si-HOTAIR + OE-DNMT1 groups. *P < 0.05 compared with the control group. l TUNEL staining was conducted in HPVEC from the control, CSE, CSE + Si-HOTAIR, CSE + Vector and CSE + Si-HOTAIR + OE-DNMT1 groups. Scale bar = 25 μm. m Statistical analysis of the AI in different groups. Results are expressed as mean ± SD. *P < 0.05. n Methylation-specific PCR (MSP) was conducted using HPVEC from the control, CSE, CSE + Si-HOTAIR, CSE + Vector and CSE + Si-HOTAIR + OE-DNMT1 groups. *P < 0.05 compared with the control group