Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primer sequences for RT-qPCR validation
| Gene | Forward primer (5’ → 3’) | Reverse primer (5’ → 3’) |
|---|---|---|
| VNN1 | TCTGCAGTGGTGAACTGGAC | GTCAAATGCCCCTAGAGCGT |
| VNN2 | GTGCTACTTACCGAAATTCATC | TTTACCAAACGCCCATCTT |
| VNN3* | GATCATTCTAAGTGGGAGTCA | CGTCCATCTCTTGAAATCTCA |
| IL1B | AGCTACGAATCTCCGACCAC | CGTTATCCCATGTGTCGAAGAA |
| GAPDH | GGAAGATGGTGATGGGATT | GGATTTGGTCGTATTGGG |
*VNN3, VNN3P Vanin 3, pseudogene (Homo sapiens)
