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. 2022 Dec 17;20:285. doi: 10.1186/s12915-022-01481-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Workflow for the construction of an ordered collection of B. theta VPI-5482 transposon mutants. Single cells from a random pool of >300,000 mutants [30] were sorted into 302 96-well plates using FACS to create a progenitor collection. After using barcode sequencing to locate strains within the progenitor collection, representative mutants for individual genes were re-arrayed to create a condensed collection of 34 96-well plates covering >2500 genes. To demonstrate the screening potential of this collection, growth and cell shape phenotypes of each strain were characterized during growth in rich medium (BHIS)