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. 2001 Dec 15;29(24):5207–5215. doi: 10.1093/nar/29.24.5207

Figure 1.

Figure 1

Figure 1

Stabilization of the exogenously and endogenously expressed p53 by TR sequences. (A) H1299 cells were transiently co-transfected with p53 expressing plasmid in the presence of pBlueScript, pBlueScript-TEL or their mixture as indicated in the table. Constant amount of DNA (10 µg/transfection) was maintained. Twenty-four hours later, the p53 protein level was determined by western blotting using DO-1 monoclonal antibody. Comparable loading is demonstrated by β-tubulin blotting. (B) Stabilization of endogenously expressed p53 by TR sequences. HepG2 (a, upper panels) or MCF-7 (b, lower panel) cells were transiently transfected with 20 µg of either pBlueScript or pBlueScript-TEL. Forty-eight hours later, p53 levels were determined by western blotting with the indicated anti-p53 monoclonal antibodies. NT, non-treated cells.