Figure 3.

Identification of the p53 protein domain required for TR induced stabilization. H1299 cells were transiently co-transfected with different p53 expressing plasmids in the presence of pBlueScript, pBlueScript-TEL as indicated in the table. Constant amount of DNA (10 µg/transfection) was maintained. Twenty-four hours later, the p53 protein level was determined by western blotting using DO-1 monoclonal antibody. Comparable loading is demonstrated by β-tubulin blotting.