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. 2022 Mar 18;207:114169. doi: 10.1016/j.bios.2022.114169

Fig. 2.

Fig. 2

MULAN detection for WT and the D614G mutant of SARS-CoV-2. (a) Schematics of MULAN detection for WT and the mutant in a one-pot reaction. The bubbles between the secondary gDNA and reporter represent the mismatches on designed gDNAs. LoD assay for the detection using template of the WT plasmid (b) and the D614G plasmid (c). Fluorescence detection of WT (d) and the D614G (e) mutant with different mutation frequencies. Error bars indicate the standard deviation of three replicates. “NTC” refers to non-template control.