Fig. 3.

Triplex MULAN detection of SARS-CoV-2, influenza A, and influenza B. LoD assay of triplex MULAN detection using diluted SARS-CoV-2 (a), influenza A (b), and influenza B (c) templates, respectively. Error bars indicate the standard deviation of three replicates. (d) Triplex MULAN detection with mixed samples of three target plasmids. The depth of red in the heat map represents the average fluorescence intensity of three replicates. (e) Triplex MULAN detection and commercial RT-qPCR test of SARS-CoV-2 pseudoviruses. The vertical dotted line represents the threshold of RT-PCR (Ct value < 38 was determined to be positive), and the horizontal dotted line represents the threshold of MULAN (the mean of the normalized fluorescence from samples with water input for three replicates). (f) Triplex MULAN detection of clinical samples with influenza viruses compared to commercial RT-qPCR test. S1–S33 refer to clinical samples. “PC” and “NTC” refer to the positive control and non-template control, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)