Table 2.

Primers used for Methylation-specific polymerase chain reaction

Step.	Gene of promoter	Forward primer	Reverse primer	annealing temperature	PCR product
Stage I	P16	GAAGAAAGAGGAGGGGTTGG	CTACAAACCCTCTACCCACC	60°C	280
	MGMT	GGATATGTTGGGATAGTT	CCAAAAACCCCAAACCC	52°C	289
	RASSF1A	GGAGGGAAGGAAGGGTAAGG	CAACTCAATAAACTCAAACTCCC	58°C	260
Stage II	P16 M	TTATTAGAGGGTGGGGCGGATCGC	GACCCCGAACCGCGACCGTAA	65°C	150
	P16 U	TTATTAGAGGGTGGGGTGGATTGT	CAACCCCAAACCACAACCATAA	65°C	151
	MGMT M	TTTCGACGTTCGTAGGTTTTCGC	GCACTCTTCCGAAAACGAAACG	59°C	81
	MGMT U	TTTGTGTTTTGATGTTTGTAGGTTTTTGT	AACTCCACACTCTTCCAAAAACAAAACA	59°C	93
	RASSF1A M	GGGGGTTTTGCGAGAGCGC	CCCGATTAAACCCGTACTTCG	65°C	204
	RASSF1A U	GGTTTTGTGAGAGTGTGTTTAG	ACACTAACAAACACAAACCAAAC	65°C	170