TABLE 1.
Virulence traits of 16 rectal-urine E. coli strains from eight dogs with UTIa
| Strain name | O antigenb | OMP pattern, clonal groupb | Agglutination patternb | No. of pap RFLP bandsb | Pilin size (kDa)b | F type (papA allele) | papc | papG allele(s)d | Presence of:
|
PAI marker | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| sfa-foc | sfaS | fyuA | hlyA | cnf1 | cdtB | kpsMT (II) | kpsMT (III) | rfc | traT | ||||||||||
| 7U | O4 | A | 6 | F10 | + | II, IIIe | + | − | + | + | + | − | + | − | + | − | + | ||
| 7R | O4 | A | NS | 6 | 19.7 | F10 | + | II, IIIe | + | − | + | + | + | − | + | − | + | − | + |
| 6U | O4 | A | NS | 5 | 19.7 | F7-2,10 | + | III | + | − | + | + | + | − | + | − | + | − | + |
| 1U | O4 | A | NS | 2 | 19.7 | F10 | + | III | + | − | + | + | + | − | − | + | + | + | + |
| 1R | O4 | A | NS | 2 | 19.7 | F10 | + | III | + | − | + | + | + | − | − | + | + | + | + |
| 3U | O120 | B | NS | 6 | 19.5 | F12,13 | + | III | + | − | + | + | + | + | − | − | − | + | + |
| 3R | O120 | B | NS | 6 | 19.5 | F12,13 | + | III | + | − | + | + | + | + | − | − | − | + | + |
| 5U | Ont | C | NS | 2 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | + | + |
| 5R | O6 | C | NS | 2 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | + | + |
| 6R | O6 | C | NS | 3 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | + | + |
| 4U | Ont | C | NS | 4 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | − | + |
| 4R | O6 | C | NS | 3 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | − | + |
| 8U | O6 | C | NS | 3 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | + | + |
| 8R | O− | C | NS | 3 | 18.7 | F48 | + | III | + | + | + | + | + | − | + | − | − | + | + |
| 2U | O149 | D | P | 2 | 18.5, 19.7 | F48 | + | II | − | − | − | − | − | − | − | − | − | + | − |
| 2R | O− | D | P | 2 | 18.5, 19.7 | F48 | + | II | − | − | − | − | − | − | − | − | − | + | − |
All strains were positive for fimH and ompT; all were negative for focG (F1C fimbrial adhesin), afaBC, bmaE (M fimbriae), gafD (G fimbriae), nfaE (nonfimbrial adhesins), iutA (aerobactin uptake), kpsMT-K1 (K1 capsule synthesis), ibeA (invasion of brain endothelium), and cvaC (colicin V). Strain name consists of dog number and source (urine [U] or rectal [R]).
As previously reported (41). Ont, O non-typeable; O−, O-antigen negative; No. of pap RFLP bands, no. of bands in Southern hybridization of pap probe with HindIII-digested genomic DNA. Clonal groups were defined by RAPD fingerprinting (Fig. 1).
All strains were positive for papAH, papC, and papEF. All strains were positive with allele II-III consensus flanking primers. Strains 7U and 7R were positive also with allele I-flanking primers. (This finding will be reported in detail separately).
papG allele was as determined by PCR using allele-specific primers internal to papG (16).
Strains 7U and 7R were subsequently found also to contain a variant of papG allele I which was not detected by published allele I-specific primers. (This finding will be reported in detail separately.)