TABLE 2.
Agglutination patterns of human and canine pap-positive strains for erythrocytes and P latex beads
| Source | Adhesin (no. of strains)b | MRHA of human RBCsa
|
Agglutination of P beadsc | |||||
|---|---|---|---|---|---|---|---|---|
| Inhibitor of A1P1 and/or OP1 RBCs
|
Neuraminidase treatment
|
Pk or p RBCs | ||||||
| PBS or BSA | Pigeon egg white | Fetuin | A1P1 RBCs | OP1 RBCs | ||||
| Human | papG allele II (2) | + | − | + | + | + | (+) | + |
| papG allele III (5) | + | − | + | + | − | − | − | |
| Sfa (1) | + | + | − | − | − | + | − | |
| Dr (1) | + | + | + | + | + | + | − | |
| Canine | papG allele II (2) | + | − | + | + | + | (+) | + |
| papG allele III (13) | + | − | + | + | − | − | − | |
For MRHA of A1P1 and OP1 RBCs, symbols are as follows: +, MRHA was as intense as positive control; −, MRHA was ≥2 intensity levels weaker than positive control (including absent MRHA). For MRHA of Pk or p RBCs, symbols are as follows: +, MRHA was as intense as positive control; (+), MRHA was 0 to 2 intensity levels weaker than positive control; −, absent MRHA. RBCs, red blood cells.
Human-source control strains included IA2 and HB101/pDC1 (papG allele II); BOS035, BOS060, BOS080, BOS117, and HB101/pJFK102 (papG allele III); HU968-298 (Sfa); and C1845 (Dr adhesins). Agglutination-positive canine strains (from present study) included 2U and 2R (papG allele II only); 1U, 1R, 3U-6R, 8U, and 8R (papG allele III only); and 7R (papG alleles II plus III, plus allele I variant). Strain 7U (papG alleles II + III, plus allele I variant) was agglutination negative.
For agglutination of P beads, presence (+) and absence (−) of agglutination is indicated.