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. 2022 Jul 31;31(24):4255–4274. doi: 10.1093/hmg/ddac170

Figure 1.

Figure 1

P0 mutant proteins analysis. (A) WB on extracts from Schwannoma cells transiently transfected with plasmids expressing predicted P0 hyperglycosylating mutants. (B) WB on extracts from Schwannoma cells transiently transfected with plasmids expressing predicted P0 hypoglycosylating mutants. (C) Confocal microscopy of Schwannoma cells transiently transfected with the EGFP-tagged hyperglycosylated P0 proteins D61N, D109N and K138N costained with the Golgi marker GM130; mutant proteins, but not P0wt, partially colocalize with GM130. Scale bar = 10 μm.