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. 2022 Jul 31;31(24):4255–4274. doi: 10.1093/hmg/ddac170

Figure 8.

Figure 8

Validation of a small selection of genes altered in the RNA-seq data. (A) WB and protein quantification (B) for E-Cadherin. (C) WB and protein quantification (D) for Laminin and (E) Cyclin B-1 in sciatic nerves of 2-month WT and MpzD61N/+ animals. Error bars, SEM; unpaired, two-tails, Student t-test; P-values of 0.0313, 0.0328 and 0.3044, respectively. One representative blot of three is shown. Quantitative RT-PCR analysis for (F) Id2 and (G) c-Jun on mRNA extracted form WT and MpzD61N/+ sciatic nerves. Error bars, SEM; unpaired, two-tails, Student t-test; P-values of 0.0202 and 0.0402, respectively; n = 5 RT from independent nerves.