Table 2.
qPCR and RT-qPCR performance characteristics and assay limit of detection (ALOD).
| Assays | qPCR/RT-qPCR performance characteristicsa |
ALOD |
||||
|---|---|---|---|---|---|---|
| Efficiency (%) | Correlation coefficient (r2) | Slope | Y-intercept | qPCR/RT-qPCR GC/reaction | GC/50 mL sample | |
| BoV | 97 | 0.99 | −3.41 | 38.38 | 7.30 | 292 |
| CmV | 106 | 1.00 | −3.18 | 37.54 | 14.2 | 568 |
| EBV | 105 | 1.00 | −3.22 | 37.54 | 5.60 | 224 |
| IAV | 95 | 1.00 | −3.45 | 38.68 | 8.40 | 336 |
| IBV | 97 | 0.97 | −3.39 | 39.21 | 7.32 | 293 |
| PeV | 99 | 0.99 | −3.33 | 38.37 | 8.10 | 324 |
| PiV 1 | 91 | 1.00 | −3.57 | 39.64 | 21.0 | 840 |
| PiV 2 | 102 | 1.00 | −3.28 | 38.01 | 17.1 | 684 |
| PiV 3 | 97 | 1.00 | −3.40 | 38.49 | 17.4 | 696 |
| RhV A | 101 | 1.00 | −3.10 | 36.13 | 9.30 | 372 |
| RhV B | 110 | 1.00 | −3.10 | 35.75 | 7.41 | 296 |
| RSV A | 103 | 0.99 | −3.26 | 37.16 | 7.32 | 293 |
| RSV B | 91 | 0.98 | −3.56 | 40.68 | 6.80 | 272 |
BoV: bocavirus; PiV 1: parainfluenza virus 1; PiV 2: parainfluenza virus 2; PiV 3: parainfluenza virus 3; PeV: parechovirus; RSV A: respiratory syncytial virus A; RSV B: respiratory syncytial virus B; RhV A: rhinovirus A; RhV B: rhinovirus B; EBV: Epstein-Barr Virus; CmV: cytomegalovirus; IAV: influenza A virus; IBV: influenza B virus.
a
Average values are presented.