Table 1.
Comparison of the analytical performance with different PCR-free strategies and techniques for the determination of HBV genome.
| Strategies | Techniques | Linear range | Sensitivity | Reference |
|---|---|---|---|---|
| HBV DNA sandwich assay | Anodic Stripping Voltammetry (ASV) | 0–500 × 108 cps μL−1 | 511 × 105 cps μL−1 | Li et al. (2015) |
| Label-free HBV DNA | Differential Pulse Voltammetry (DPV) | 50 × 108–100 × 1011 cps μL−1 | 8.71 × 105 cps μL−1 | Srisomwat et al., (2020) |
| PCR-free HBV genome detection | Square Wave Voltammetry (SWV) | 0.1–100 cps μL−1 | 1 cps μL−1a | Petralia et al., (2017) |
| Multicolors QDs based sensor | Electrochemiluminescence (ECL) | 0.0005–0.5 × 1011 cps μL−1 | 0.49 × 105 cps μL−1 | Liu et al. (2016) |
| Carbon sphere/Polyaniline | Differential Pulse Voltammetry (DPV) | 0.01 × 108–1 × 1011 cps μL−1 | 3.62 × 105 cps μL−1 | Salimian et al. (2019) |
| Label-free platform using graphene QDs | Differential Pulse Voltammetry (DPV) | 1012 - 5 × 1013 cps μL−1 | 1011 cps μL−1 | Xiang et al., (2018) |
| PCR-free EG ds-HBV | Electrochemiluminescence (ECL) | EG ds-HBV 0–0.75 ± 0.011–0.017 cps μL−1 |
EG ds-HBV 0.06 ± 0.011 cps μL−1 |
This work |
a
This LoD it was obtained using SG ds-DNA.