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. 2022 Dec 17;12:21833. doi: 10.1038/s41598-022-26020-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of morphologically normal spermatozoa originating from extended semen samples with either enhanced (≥ 15% = high MAS; n = 10 boars) or low (< 5% = low MAS; n = 9 boars) prevalence of cytoplasmic droplets and/or bent tails. Data are based on sperm tracks analysed after a 30-min incubation period at 38 °C in Beltsville Thawing Solution. (a) Motility descriptors (mean ± SD) are given for each cluster. b) Distribution of spermatozoa to the individual cluster based on their origin from high MAS samples or low MAS samples. Cramer’s V indicates a small effect of the sample on sperm distribution. (c)–(e) Comparison of sperm distribution in high MAS samples (n = 10 boars) and low MAS samples (n = 9 boars) for Cluster 1 (c), Cluster 2 (d), Cluster 3 (e) and Cluster 4 (f). Boxes show the interquartile range. Vertical lines indicate minimum and maximum, while the horizontal line in the box indicates the median. An asterisk (*) indicates a significant difference between samples (P < 0.05).