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. 2022 Dec 17;12:21833. doi: 10.1038/s41598-022-26020-5

Figure 4.

Figure 4

Motility patterns of morphologically normal spermatozoa originating from extended semen samples with either enhanced (≥ 15% = high MAS; n = 10 boars) or low (< 5% = low MAS; n = 9 boars) prevalence of sperm with cytoplasmic droplets and/or bent tails in the absence or presence of procaine. Extended semen samples were centrifuged, and pelleted sperm incubated in non-capacitating Tyrode’s medium without (control) or with 7.3 mM procaine for 10 min at 38 °C. (a) Motility characteristics of sperm from the different clusters. Only clusters containing at least 5% of sperm in any of the sperm subsets from this experiment were considered for evaluation. (b) Effect of procaine on motility patterns of sperm with normal morphology from samples with low MAS (upper pie charts) or high MAS (lower pie charts). Cramer’s V (range from 0 to 1) indicates the effect size of sample origin or procaine treatment on sperm distribution to the different cluster (movement patterns). V < 0.1 = no effect; 0.1 ≤ V < 0.3 small effect; 0.3 ≤ V < 0.5 moderate effect; V ≥ 0.5 = large effect.