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. 2022 Dec 17;12:21833. doi: 10.1038/s41598-022-26020-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Prevalence of non-viable sperm with normal tail morphology or with proximal or distal CD or bent tail during storage at 17 °C up to 168 h and subsequent thermic incubation at 38 °C for 3 h. Extended semen originated from samples with either enhanced (≥ 15% = high MAS; n = 15 boars) or low (< 5% = low MAS; n = 11 boars) prevalence of cytoplasmic droplets and/or bent tails. An asterisk (*) indicates a significant difference between sperm with a CD or bent tail and normal sperm within a time point (p < 0.05). There were no differences between normal sperm from high and low MAS samples (p > 0.05).