FIGURE 1.

Overall three‐dimensional structure of Streptococcus pyogenes Cas9‐sgRNA‐DNA ternary complex. (A) Domain organization. (B) Ribbon representation of Cas9‐sgRNA‐DNA complex at different angles, obtained from the protein data bank (https://www.rcsb.org, PDB ID: 4OO8), edited using software developed by Resource for Biocomputing, Visualization, and Informatics at University of California, San Francisco Chimera. Abbreviations: NUC, nuclease; REC, recognition; PI, PAM interacting; PAM, protospacer adjacent motif; sgRNA, single‐guide RNA