Table 3.
Statistical comparison between the results obtained by applying the proposed spectrophotometric methods and reported method for determination of DPA
| Parameters | First derivative | Ratio derivative | Ratio difference | Mean centering | Dual wavelength | Reported method [6]*** |
|---|---|---|---|---|---|---|
| Mean | 100.21 | 99.94 | 100.11 | 99.78 | 99.47 | 98.99 |
| S.D | 1.143 | 0.999 | 1.036 | 0.607 | 1.082 | 0.409 |
| N* | 5 | 5 | 5 | 5 | 5 | 5 |
| t-test** | 2.203 (2.365) | 1.931 (2.365) | 2.199 (2.365) | 2.319 (2.365) | 0.907 (2.365) | – |
| F-value** | 7.820 (9.117) | 5.984 (9.117) | 6.426 (9.117) | 2.206 (9.117) | 7.014 (9.117) | – |
*Number of experimental.
**The values in the parenthesis are the corresponding theoretical values of t and F at (P = 0.05).
***Determination of the Content of Desmopressin in Pharmaceutical Preparations by HPLC and Validation of the Method [6].