Antibodies |
|
|
|
Monoclonal mouse CD45-vioblue, clone 5B1 |
Miltenyi Biotec |
Cat#130-092-880; RRID:AB_1103220 |
Monoclonal rabbit c-MYB, clone EP769Y |
Abcam |
Cat#ab45150; RRID:AB_778878 |
Monoclonal rabbit cleaved Notch1, clone D3B8 |
Cell Signaling Technology |
Cat#4147; RRID:AB_2153348 |
Calcein AM |
ThermoFisher |
Cat#C3100MP |
TO-PRO-3 iodide |
ThermoFisher |
Cat#T3605 |
|
Biological samples |
|
|
|
Human head and neck adenoid cystic carcinoma patient samples (ACC2, ACC5, ACC7, ACC15, ACC19, ACC21, ACC22) |
Massachusetts Eye and Ear |
N/A |
|
Critical commercial assays |
|
|
|
Human Tumor Dissociation Kit |
Miltenyi Biotec |
Cat#130-095-929 |
BOND Polymer Refine Detection Kit |
Leica |
Cat#DS9800 |
|
Deposited data |
|
|
|
Processed scRNA-seq data |
GEO |
GSE210171 |
Raw scRNA-seq |
dbGaP |
phs003070.v1 |
|
Software and algorithms |
|
|
|
MatLab version 2019b |
MathWorks |
N/A |
STAR version 2.5.2 |
https://github.com/alexdobin/STAR
|
N/A |
Seurat version 4.0.1 |
https://github.com/satijalab/seurat
|
N/A |
inferCNV version 1.4.0 |
https://bioconductor.org/packages/release/bioc/html/infercnv.html
|
N/A |
cellphoneDB version 4 |
https://github.com/ventolab/CellphoneDB
|
N/A |