Table 3.

Statistical Analysis of Neutrophils data from Mouse Air Pouch Model

A. Raw Data: Neutrophils
Total Cell (x1,000)
Mouse Status		3D			7D
		N	Mean	SD	N	Mean	SD
Non-diabetic	S	8	29	5	14	38	14
	D	8	60	64	10	106	52
Diabetic	S	7	37	7	5	31	16
	I	5	86	38	5	116	40
	D	6	103	56	8	97	75

B. S vs. D
P value from two-sample t-tests
Non-Diabetic	3D	NS
Non-Diabetic	7D	<0.01

C. S vs. D vs. I
P value from one-way ANOVA and Tukey post-hoc adjustment
		S vs. D. vs. I	D vs. I	I vs. S	D vs. S
Diabetic	3D	<0.02	NS	NS	<0.02
Diabetic	7D	NS	NS	NS	NS

D. 3D vs. 7D
P value from two-sample t-tests
Non-Diabetic	S	<0.05
Non-Diabetic	D	NS
Diabetic	S	NS
Diabetic	D	NS
Diabetic	I	NS

E. Non-diabetic vs. Diabetic
P value from two-sample t-tests
S	3D	<0.02
S	7D	NS
D	3D	NS
D	7D	NS

^A)Data represents n-value, mean, and standard deviation (SD) for total Neutrophil recruitment following infusion of saline (S), diluent (D), and insulin (I) for 3-days (3D) and 7-days (7D) in non-diabetic and diabetic mice;

^B)p-values for infusion of S versus D for 3D and 7D in non-diabetic animals;

^C)p-values for infusion of S, D, and I for 3D and 7D in diabetic animals;

^D)p-values for 3D versus 7D infusion of S, D, I in non-diabetic and diabetic animals;

^E)p-values for non-diabetic versus diabetic infusion of S and D for 3D and 7D.