Table 4.

Statistical Analysis for Macrophage/Monocyte data from Mouse Air Pouch Model

A. Raw Data: Macrophages/Monocytes
Total Cell (x1,000)
Mouse Status		3D			7D
		N	Mean	SD	N	Mean	SD
Non-diabetic	S	8	27	24	14	12	6
	D	8	127	66	10	64	34
Diabetic	S	7	10	4	5	15	9
	I	5	136	64	5	71	46
	D	6	100	49	8	64	40

B. S vs. D
P value from two-sample t-tests
Non-Diabetic	3D	<0.01
Non-Diabetic	7D	<0.001

C. S vs. D vs. I
P value from one-way ANOVA and Tukey post-hoc adjustment
		S vs. D. vs. I	D vs. I	I vs. S	D vs. S
Diabetic	3D	<0.001	NS	<0.001	<0.01
Diabetic	7D	<0.05	NS	NS	NS

D. 3D vs. 7D
P value from two-sample t-tests
Non-Diabetic	S	NS
Non-Diabetic	D	<0.02
Diabetic	S	NS
Diabetic	D	NS
Diabetic	I	NS

E. Non-diabetic vs. Diabetic
P value from two-sample t-tests
S	3D	NS
S	7D	NS
D	3D	NS
D	7D	NS

^A)Data represents n-value, mean, and standard deviation (SD) for total Macrophage/Monocyte recruitment following infusion of saline (S), diluent (D), and insulin (I) for 3-days (3D) and 7-days (7D) in non-diabetic and diabetic mice;

^B)p-values for infusion of S versus D for 3D and 7D in non-diabetic animals;

^C)p-values for infusion of S, D, and I for 3D and 7D in diabetic animals;

^D)p-values for 3D versus 7D infusion of S, D, I in non-diabetic and diabetic animals;

^E)p-values for non-diabetic versus diabetic infusion of S and D for 3D and 7D.