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. 2023 Jan 1;19(1):89–103. doi: 10.7150/ijbs.74227

Figure 3.

Figure 3

Overexpression of miR-1275 reduced CSCs properties in in vitro. A. The expression levels of CD44 and CD24 in cells after transfection were analyzed by flow cytometry analysis. Cells were stained with anti-CD44-APC and anti-CD24-PE. B. Spheroids formation assay was performed, and the representative pictures were shown. C. Protein expression of stem-related genes such as CD44, CD133, NANOG, OCT4, SOX2 and BMI1 in MCF-7, MCF-7/ADR, MDA-MB-231 and SUM-1315. D. mRNA expression of stem-related genes such as CD44, CD133, NANOG, OCT4, SOX2 and BMI1 in MCF-7, MCF-7/ADR, MDA-MB-231 and SUM-1315. *p < 0.05, **p<0.01, ***p < 0.001. The data expressed as the mean ± SD. Scale bar, 100 µm.