Skip to main content
. 2023 Jan 1;19(1):89–103. doi: 10.7150/ijbs.74227

Figure 4.

Figure 4

MDK was a direct downstream target of miR-1275. A. Venn diagram of predicted miR-1275 targets by 4 programs (TargetScan, miRDB, miRwalk and DIANA). B. The 3'-end biotin-labeled miR-1275 mimics and negative control were transfected into MCF-7/ADR and MDA-MB-231 cells, and the mRNA levels of MDK, COL1A2, F2RL3 and GAPDH were quantified by RT-qPCR, and the relative immunoprecipitate/input ratios were plotted. C. The luciferase reporter constructed that had either a MDK-WT or MDK-MUT sequence of the miR-1275 binding site. D. The luciferase reporter containing wild-type MDK-WT or MDK- MUT was transfected into BC cells correspondingly. Luciferase activity was determined by the dual luciferase assay. E. The mRNA level of MDK in BC cells after the transfection of miR-1275 were determined with RT-qPCR. F. The protein expression levels of MDK in cell lysate and conditioned media in BC cells after the transfection of miR-1275 were determined with western blot. G. The protein expression levels of MDK in conditioned media in BC cells after the transfection of miR-1275 were determined with ELISA assay. *p < 0.05, **p<0.01, ***p < 0.001. The data expressed as the mean ± SD.